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Single-Round, Multiplexed Antibody Mimetic Design through mRNA Display


  • Funding for this work was provided by the NIH, 5R01AI085583 (R.W.R., H.T.S., and R.S.). C.A.O. was supported by the NCI Cancer Education Grant, R25 CA 098010. J.A.T was supported by the Charlotte Geyer Foundation and the Morgridge Institute for Research.


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In a single round: By combining the high-efficiency enrichment through the continuous-flow magnetic separation (CFMS) technique with the analytical power of next-generation sequencing, the generation of antibody mimetics with a single round of mRNA display is made possible. This approach eliminates iterative selection cycles and provides a path to fully automated ligand generation (see picture).