DNA Aptamer-Mediated Cell Targeting

Authors

  • Xiangling Xiong,

    1. Departments of Chemistry, of Physiology and Functional Genomics, of Molecular Genetics and Microbiology, and of Pathology and Laboratory Medicine, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200 (USA)
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  • Dr. Haipeng Liu,

    1. Departments of Chemistry, of Physiology and Functional Genomics, of Molecular Genetics and Microbiology, and of Pathology and Laboratory Medicine, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200 (USA)
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  • Dr. Zilong Zhao,

    1. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (P.R. China)
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  • Dr. Meghan B. Altman,

    1. Departments of Chemistry, of Physiology and Functional Genomics, of Molecular Genetics and Microbiology, and of Pathology and Laboratory Medicine, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200 (USA)
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  • Dr. Dalia Lopez-Colon,

    1. Departments of Chemistry, of Physiology and Functional Genomics, of Molecular Genetics and Microbiology, and of Pathology and Laboratory Medicine, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200 (USA)
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  • Prof. Chaoyong James Yang,

    Corresponding author
    1. State Key Laboratory for Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
    • State Key Laboratory for Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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  • Prof. Lung-Ji Chang,

    1. Departments of Chemistry, of Physiology and Functional Genomics, of Molecular Genetics and Microbiology, and of Pathology and Laboratory Medicine, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200 (USA)
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  • Prof. Chen Liu,

    1. Departments of Chemistry, of Physiology and Functional Genomics, of Molecular Genetics and Microbiology, and of Pathology and Laboratory Medicine, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200 (USA)
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  • Prof. Weihong Tan

    Corresponding author
    1. Departments of Chemistry, of Physiology and Functional Genomics, of Molecular Genetics and Microbiology, and of Pathology and Laboratory Medicine, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200 (USA)
    2. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082 (P.R. China)
    • Departments of Chemistry, of Physiology and Functional Genomics, of Molecular Genetics and Microbiology, and of Pathology and Laboratory Medicine, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200 (USA)
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  • This work is supported by grants awarded by the National Institutes of Health (GM066137, GM079359 and CA133086), by the National Key Scientific Program of China (2011CB911000), the China National Instrumentation Program (2011YQ03012412), and the Foundation for Innovative Research Groups of NSFC (21221003).

Abstract

original image

An apt modification: A simple and effective way to modify the cell surface with target-specific ligands, such as DNA aptamers, while minimizing the effects on the modified cells has been developed. After incubating with lipo–aptamer probes, immune cells (red, see scheme) recognize and kill cancer cells (blue) in the cell mixture.

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