Tracking the Bioreduction of Disulfide-Containing Cationic Dendrimers

Authors

  • Lorine Brülisauer,

    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
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  • Nadia Kathriner,

    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
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  • Mark Prenrecaj,

    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
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  • Dr. Marc A. Gauthier,

    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
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  • Prof. Dr. Jean-Christophe Leroux

    Corresponding author
    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
    • Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
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  • L. B. acknowledges the Scholarship Fund of the Swiss Chemical Industry (2-70882-08). The Light Microscopy Center (ETHZ) and Alois Renn are thanked for experimental support. Ari Helenius kindly provided RFP-Rab5 plasmids.

Abstract

original image

Disulfides enhance the transfection efficacy and reduce the toxicity of cationic gene delivery polymers. A quantitative analysis is provided of the bioreduction of a dynamic bioreducible dendritic polycationic probe in four cell lines. Such knowledge is indispensible for understanding and optimizing bioreducible drug and gene delivery systems.

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