Get access

Tracking the Bioreduction of Disulfide-Containing Cationic Dendrimers

Authors

  • Lorine Brülisauer,

    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
    Search for more papers by this author
  • Nadia Kathriner,

    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
    Search for more papers by this author
  • Mark Prenrecaj,

    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
    Search for more papers by this author
  • Dr. Marc A. Gauthier,

    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
    Search for more papers by this author
  • Prof. Dr. Jean-Christophe Leroux

    Corresponding author
    1. Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
    • Swiss Federal Institute of Technology Zurich (ETHZ), Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, Wolfgang-Pauli-Strasse 10, 8093 Zurich (Switzerland)
    Search for more papers by this author

  • L. B. acknowledges the Scholarship Fund of the Swiss Chemical Industry (2-70882-08). The Light Microscopy Center (ETHZ) and Alois Renn are thanked for experimental support. Ari Helenius kindly provided RFP-Rab5 plasmids.

Abstract

original image

Disulfides enhance the transfection efficacy and reduce the toxicity of cationic gene delivery polymers. A quantitative analysis is provided of the bioreduction of a dynamic bioreducible dendritic polycationic probe in four cell lines. Such knowledge is indispensible for understanding and optimizing bioreducible drug and gene delivery systems.

Get access to the full text of this article

Ancillary