Get access
Advertisement

Reconstitution of Pole-to-Pole Oscillations of Min Proteins in Microengineered Polydimethylsiloxane Compartments

Authors

  • Katja Zieske,

    1. Dept. Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried (Germany)
    Search for more papers by this author
  • Prof. Dr. Petra Schwille

    Corresponding author
    1. Dept. Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried (Germany)
    • Dept. Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried (Germany)

    Search for more papers by this author

  • We thank Helge Vogl for technical advice and Zdenek Petrášek for comments on the manuscript. This work has been financially supported by the DFG Center of Excellence “Nanosystems Initiative Munich” (NIM), the SFB 1032, and the ESF EuroSYNBIO/SYNDIV. K.Z. is supported by “The International Max Planck Research School for Molecular and Cellular Life Sciences” (IMPRS-LS).

Abstract

original image

Cell division in bacteria is highly regulated in time and space. The use of micrometer-sized sample volumes and model membranes allows the pole-to-pole oscillations of spatial regulators for bacterial cell division to be reconstituted in a synthetic minimal system (see schematic picture left as well as the confocal images of Min proteins on lipid membranes in a polydimethylsiloxane microcompartment right).

Get access to the full text of this article

Ancillary