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Communication

Red-Light-Induced Inhibition of DNA Replication and Amplification by PCR with an Os/Rh Supramolecule†

  1. Jing Wang1,
  2. Jerry Newman Jr.1,
  3. Dr. Samantha L. H. Higgins1,
  4. Kaitlyn M. Brewer1,
  5. Prof. Brenda S. J. Winkel2,
  6. Prof. Karen J. Brewer1,*

Article first published online: 6 DEC 2012

DOI: 10.1002/anie.201207083

Issue

Angewandte Chemie International Edition

Angewandte Chemie International Edition

Volume 52, Issue 4, pages 1262–1265, January 21, 2013

Additional Information

How to Cite

Wang, J., Newman, J., Higgins, S. L. H., Brewer, K. M., Winkel, B. S. J. and Brewer, K. J. (2013), Red-Light-Induced Inhibition of DNA Replication and Amplification by PCR with an Os/Rh Supramolecule . Angew. Chem. Int. Ed., 52: 1262–1265. doi: 10.1002/anie.201207083

Author Information

  1. 1

    Department of Chemistry, Virginia Tech, Blacksburg, VA 24061-0212 (USA)

  2. 2

    Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061-0212 (USA)

*Department of Chemistry, Virginia Tech, Blacksburg, VA 24061-0212 (USA)

  1. †

    Acknowledgements are provided to Virginia Tech for support of this work.

Publication History

  1. Issue published online: 15 JAN 2013
  2. Article first published online: 6 DEC 2012
  3. Manuscript Received: 31 AUG 2012

Funded by

  • Virginia Tech

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Keywords:

  • DNA amplification;
  • metal complexes;
  • photochemistry;
  • photodynamic therapy;
  • polymerase chain reaction
Thumbnail image of graphical abstract

Bimetallic: Upon irradiation with light in the phototherapeutic window, the bimetallic complex [(bpy)2Os(dpp)RhCl2(phen)]3+ binds to and cleaves DNA through an oxygen-independent mechanism. PCR is used as a model for in vitro DNA replication. The results show that the metal complex does not interfere with DNA amplification in the dark without O2, but completely inhibits DNA amplification in samples exposed to red light. Taq=polymerase, PS=photosensitizer.

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