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  • Open Access

2′-SCF3 Uridine—A Powerful Label for Probing Structure and Function of RNA by 19F NMR Spectroscopy

Authors

  • M. Sc. Katja Fauster,

    1. Institute of Organic Chemistry (IOC) and Center for Molecular Biosciences (CMBI), University of Innsbruck, Center for Chemistry and Biomedicine (CCB), 6020 Innsbruck (Austria)
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  • Dr. Christoph Kreutz,

    1. Institute of Organic Chemistry (IOC) and Center for Molecular Biosciences (CMBI), University of Innsbruck, Center for Chemistry and Biomedicine (CCB), 6020 Innsbruck (Austria)
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  • Prof. Dr. Ronald Micura

    Corresponding author
    1. Institute of Organic Chemistry (IOC) and Center for Molecular Biosciences (CMBI), University of Innsbruck, Center for Chemistry and Biomedicine (CCB), 6020 Innsbruck (Austria)
    • Institute of Organic Chemistry (IOC) and Center for Molecular Biosciences (CMBI), University of Innsbruck, Center for Chemistry and Biomedicine (CCB), 6020 Innsbruck (Austria)
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  • We thank Prof. Dr. A. Serganov (NYULMC) for the generous gift of U1A protein, Priv.-Doz. Dr. K. Breuker for FT-ICR mass spectra, Dr. B. Puffer for initial experiments on 2′-OCF3 uridine, M. Sc. S. Grutsch and M. Sc. T. Moschen for preparation of E. coli lysate, and Dr. M. Soulière for critical comments. We are grateful for funding from the Austrian Science Fund FWF (I317, P21641 to R.M.; I844 to C.K.).

Abstract

original image

Fluorishing: The Togni reagent allows efficient synthetic access to fluorine-labeled RNA molecules (see picture). These are in turn highly useful for NMR spectroscopic analyses of secondary and tertiary structures, RNA–protein interactions, and functionality of riboswitch modules.

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