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Probing the Function of the Tyr-Cys Cross-Link in Metalloenzymes by the Genetic Incorporation of 3-Methylthiotyrosine

Authors

  • Qing Zhou,

    1. Laboratory of Non-coding RNA„ Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
    2. Graduate University of the Chinese Academy of Sciences, Beijing 100864 (China)
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    • These authors contributed equally to this work.

  • Dr. Meirong Hu,

    1. Laboratory of Non-coding RNA„ Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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    • These authors contributed equally to this work.

  • Dr. Wei Zhang,

    1. Laboratory of Non-coding RNA„ Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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    • These authors contributed equally to this work.

  • Dr. Li Jiang,

    1. Laboratory of Non-coding RNA„ Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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  • Prof. Dr. Sarah Perrett,

    1. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing (China)
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  • Juanzuo Zhou,

    1. Laboratory of Non-coding RNA„ Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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  • Prof. Dr. Jiangyun Wang

    Corresponding author
    1. Laboratory of Non-coding RNA„ Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
    • Laboratory of Non-coding RNA„ Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101 (China)
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  • We gratefully acknowledge the Major State Basic Research Program of China (2010CB912301, 2009CB825505), the National Science Foundation of China (90913022, 30900252, 30900231), and CAS grants (KZCX2-YW-JC101 and KSCX2-EW-G-7) to J.W. We thank Z. Xie and X. Peng for help with protein mass spectrometry.

Abstract

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Efficient multiple-electron redox reactions are performed by galactoase oxidase, cysteine dioxygenase, sulfite reductase, and cytochrome c nitrite reductase. A post-translationally modified tyrosine–cysteine cross-link (Tyr-Cys) in their active sites is essential for their functions.

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