Quantifying Analytes in Paper-Based Microfluidic Devices Without Using External Electronic Readers


  • This work was supported by the Arnold and Mabel Beckman Foundation, the Camille and Henry Dreyfus Foundation, 3M, and Louis Martarano. S.T.P. acknowledges support from the Alfred P. Sloan Research Fellows program. We thank Matthew S. Baker for assistance with experiments and Landy K. Blasdel for assistance with the manuscript.


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A matter of time: Two quantitative assays are presented that require only measurements of time and the ability to see and/or count to quantify the presence of a specific analyte. The strategies rely on specific changes in wetting properties of paper when a hydrophobic detection reagent (compound 1) reacts with a desired analyte to produce hydrophilic products. This change in wetting properties enables selective and quantitative detection of the analyte.