Get access

Metabolic Differences in Microbial Cell Populations Revealed by Nanophotonic Ionization


  • Support from the Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the U.S. Department of Energy (Grant DE-FG02-01ER15129) is gratefully acknowledged. A portion of this research (nanofabrication) was conducted at the Center for Nanophase Materials Sciences, which is sponsored at Oak Ridge National Laboratory by the Scientific User Facilities Division, Office of Basic Energy Sciences, U.S. Department of Energy.


original image

Playing (yeast) laser tag: Laser desorption ionization mass spectrometry (LDI-MS) with nanopost arrays (NAPA, see scheme) was shown to quantitatively analyze trace amounts of intracellular metabolites from single cells of Saccharomyces cerevisiae (baker's yeast). The upregulation and downregulation of metabolites because of external stress (such as oxidative stress) are also observed, even at the single-cell level.

Get access to the full text of this article