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Complete Oxidation of Methanol in Biobattery Devices Using a Hydrogel Created from Three Modified Dehydrogenases

Authors


  • This work was funded by the NSF and the AFOSR. We thank Prof. Prather at MIT for the FDH1 plasmid peAF and Prof. Wood at Princeton University for the intein gene contained in plasmid pE1OPD. We also thank Hoang D. Lu for technical assistance.

Abstract

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Enzyme catalysis: Three dehydrogenases have been engineered to self-assemble into a hydrogel that supports a synthetic metabolic network. The new catalytic biomaterial was used as an anode modification in two enzymatic biobatteries capable of the complete oxidation of methanol to CO2 (see picture).

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