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Reversible Sensing of the Anticoagulant Heparin with Protamine Permselective Membranes

Authors

  • Gastón A. Crespo,

    1. Department of Inorganic, Analytical and Applied Chemistry, University of Geneva, Quai E. Ansermet 30, 1211 Geneva (Switzerland)
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  • Majid Ghahraman Afshar,

    1. Department of Inorganic, Analytical and Applied Chemistry, University of Geneva, Quai E. Ansermet 30, 1211 Geneva (Switzerland)
    2. On leave from Imam Khomeini International University, Qazvin (Iran)
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  • Prof. Eric Bakker

    Corresponding author
    1. Department of Inorganic, Analytical and Applied Chemistry, University of Geneva, Quai E. Ansermet 30, 1211 Geneva (Switzerland)
    • Department of Inorganic, Analytical and Applied Chemistry, University of Geneva, Quai E. Ansermet 30, 1211 Geneva (Switzerland)
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  • The authors thank the Swiss National Science Foundation and the University of Geneva for supporting this research, and Dr. Till Saxer and Dr. Karim Bendjelid (HUG) for collaborating on this project and for providing human blood bags. M.G.A. thanks Imam Khomeini International University for an international fellowship.

Abstract

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A permselective membrane electrode allows the rapid and operationally reversible detection of the polycationic polypeptide protamine in physiological samples. Anticoagulant levels of heparin can be measured in undiluted whole blood by adding a known excess of its antidote protamine to discrete blood samples.

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