These authors contributed equally to this work.
Homologous NRPS-like Gene Clusters Mediate Redundant Small-Molecule Biosynthesis in Aspergillus flavus†
Article first published online: 20 DEC 2012
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 5, pages 1590–1594, January 28, 2013
How to Cite
Forseth, R. R., Amaike, S., Schwenk, D., Affeldt, K. J., Hoffmeister, D., Schroeder, F. C. and Keller, N. P. (2013), Homologous NRPS-like Gene Clusters Mediate Redundant Small-Molecule Biosynthesis in Aspergillus flavus . Angew. Chem. Int. Ed., 52: 1590–1594. doi: 10.1002/anie.201207456
We thank J. A. Baccile, Y. Rokhlenko, and J. A. Bernstein for assistance with synthesis and M. Kukula for help with mass spectrometry. Support from the U.S. National Institutes of Health (GM008500 to R.R.F. and GM084077 to N.P.K.), the International Leibniz Research School Jena (to D.S.), DuPont Corp. (to F.C.S.), the U.S. Department of Agriculture (WIS01200 to N.P.K.), and the Food Research Institute, National Science Foundation subagreement (IOS-0965649 to N.P.K) is gratefully acknowledged.
- Issue published online: 23 JAN 2013
- Article first published online: 20 DEC 2012
- Manuscript Revised: 8 NOV 2012
- Manuscript Received: 15 SEP 2012
- National Institutes of Health. Grant Numbers: GM008500, GM084077
- International Leibniz Research School Jena
- U.S. Department of Agriculture. Grant Number: WIS01200
- Food Research Institute, National Science Foundation. Grant Number: IOS-0965649
- comparative metabolomics;
- genome mining;
- natural products;
- secondary metabolism
Biosynthetic crosstalk: Most gene clusters in fungi are orphans with no known associated metabolites. NMR-based comparative metabolomics was used to identify the products of two highly homologous orphan clusters in Aspergillus flavus. The two clusters encode partially redundant biosynthetic pathways that produce overlapping sets of novel fungal alkaloids, feature NRPS-like genes with unusual functions, and regulate fungal development.