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A Ratiometric Fluorescent Probe for Rapid Detection of Hydrogen Sulfide in Mitochondria

Authors

  • Yuncong Chen,

    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • Chengcheng Zhu,

    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • Zhenghao Yang,

    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • Junjie Chen,

    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • Yafeng He,

    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • Yang Jiao,

    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • Prof. Weijiang He,

    Corresponding author
    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
    • State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • Lin Qiu,

    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • Jiajie Cen,

    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • Prof. Zijian Guo

    Corresponding author
    1. State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
    • State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)
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  • We thank the National Basic Research Program of China (No. 2011CB935800) and National Natural Science Foundation of China (No. 21271100, 91213305, 10979019, 21131003, and 21021062) for financial support.

Abstract

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Quick: An exogenously induced quick increase of the H2S concentration (80 s) in MCF-7 cells can be visualized by ratiometric imaging using a new probe (CouMC) that can target mitochondria. CouMC was constructed by combining merocyanine and coumarin fluorophores. The selective nucleophilic addition of HS to the merocyanine derivative at neutral pH is crucial for the rapid H2S detection.

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