These authors contributed equally to this work.
Novel Activity-Based Probes for Broad-Spectrum Profiling of Retaining β-Exoglucosidases In Situ and In Vivo†
Article first published online: 8 NOV 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 51, Issue 50, pages 12529–12533, December 7, 2012
How to Cite
Kallemeijn, W. W., Li, K.-Y., Witte, M. D., Marques, A. R. A., Aten, J., Scheij, S., Jiang, J., Willems, L. I., Voorn-Brouwer, T. M., van Roomen, C. P. A. A., Ottenhoff, R., Boot, R. G., van den Elst, H., Walvoort, M. T. C., Florea, B. I., Codée, J. D. C., van der Marel, G. A., Aerts, J. M. F. G. and Overkleeft, H. S. (2012), Novel Activity-Based Probes for Broad-Spectrum Profiling of Retaining β-Exoglucosidases In Situ and In Vivo . Angew. Chem. Int. Ed., 51: 12529–12533. doi: 10.1002/anie.201207771
This work was supported by The Netherlands Organization for Scientific Research (NWO-CW) and The Netherlands Genomics Initiative. We thank K. T. Senel for technical assistance.
- Issue published online: 4 DEC 2012
- Article first published online: 8 NOV 2012
- Manuscript Received: 26 SEP 2012
- The Netherlands Organization for Scientific Research (NWO-CW)
- The Netherlands Genomics Initiative
- activity-based probes;
- fluorescent probes;
A high-end label: Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases (see picture). These probes offer new ways to study β-exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity-based probes targeting other retaining glycosidase families.