The Importance of Additive and Non-Additive Mutational Effects in Protein Engineering


  • I thank all past and present co-workers who have participated in discussions regarding non-additive effects, especially J. D. Carballeira, J. Sanchis, C. Acevedo-Rocha, and S. Kille, as well as Prof. Yuval Nov (Haifa/Israel) for helpful comments. Support by the Max-Planck-Society and the Arthur C. Cope foundation is gratefully acknowledged.


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Additive or non-additive, that is the question: The increasing awareness of non-additive cooperative mutational effects in protein engineering is currently providing new theoretical and practical insights. In particular, the directed evolution of stereoselective enzymes as catalysts in organic chemistry and biotechnology profits from this intriguing development.