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Non-covalent Single Transcription Factor Encapsulation Inside a DNA Cage

Authors

  • Dr. Robert Crawford,

  • Dr. Christoph M. Erben,

    1. Biological Physics Research Group, Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford, OX1 3PU (UK)
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  • Dr. Javier Periz,

    1. Biological Physics Research Group, Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford, OX1 3PU (UK)
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  • Dr. Lucy M. Hall,

    1. School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ (UK)
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  • Prof. Tom Brown,

    1. School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ (UK)
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  • Prof. Andrew J. Turberfield,

    1. Biological Physics Research Group, Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford, OX1 3PU (UK)
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  • Dr. Achillefs N. Kapanidis

    Corresponding author
    1. Biological Physics Research Group, Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford, OX1 3PU (UK)
    • Biological Physics Research Group, Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford, OX1 3PU (UK)===

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  • We thank Dr. Louise Aigrain for helpful discussions. R.C. was supported by the Life Sciences Interface Doctoral Training Centre and Linacre College, University of Oxford. A.N.K. was supported by a European Commission Seventh Framework Program grant (FP7/2007-2013 HEALTH-F4-2008-201418), a UK BBSRC grant (BB/H01795X/1), and a European Research Council grant (261227). L.M.H. was funded by a UK BBSRC Ph.D. studentship. A.J.T. was supported by UK EPSRC grant EP/G037930/1 and a Royal Society-Wolfson Research Merit Award.

Abstract

original image

CAP-turing the moment: A cage made from DNA can be used to encapsulate a transcription factor (catabolite activator protein, CAP; see figure) without covalent attachment. CAP is shown to stably bind inside the DNA cage at a 1:1 ratio by bending one edge to accommodate the protein. Single-molecule fluorescence measurements confirm the orientation of CAP within the cage.

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