Triple-Helical Recognition of RNA Using 2-Aminopyridine-Modified PNA at Physiologically Relevant Conditions


  • We thank Binghamton University and the NIH (R01 GM071461) for support of this research. The Regional NMR Facility (600 MHz instrument) at Binghamton University is supported by the NSF (CHE-0922815).


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Peptide nucleic acids containing thymidine and 2-aminopyridine (M) nucleobases form stable and sequence-selective triple helices with double-stranded RNA at physiologically relevant conditions. The M-modified PNA showed unique RNA selectivity by having two orders of magnitude higher affinity for the double-stranded RNAs than for the same DNA sequences.