Combinatorial Nucleoside-Deletion-Scanning Mutagenesis of Functional DNA


  • We are grateful to the Max Planck Society and the MPI for biophysical chemistry for the generous support of our research. Members of the research group Nucleic Acid Chemistry are gratefully acknowledged for stimulating discussions. We thank Uwe Plessmann and Jürgen Bienert from the chemistry and mass spectrometry facilities at MPIbpc for recording NMR and MS spectra.


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Compact and informative: Individual nucleosides are statistically mutated by replacement with a non-nucleosidic spacer unit Δ that encodes the “deletion” in a single DNA library. This efficient mutagenesis approach enables minimization of functional DNA, identifying essential nucleotides, as demonstrated for two nucleic-acid ligating deoxyribozymes.