Supramolecular Control of Enzyme Activity through Cucurbit[8]uril-Mediated Dimerization


  • Part of the Ph.D. thesis of D.T.D. Dr. T. Reubold and Dr. S. Eschenburg (Max Planck Institute of Molecular Physiology, Dortmund (Germany)) are thanked for kindly providing the DNA of caspases-3 and 9. Wencke Adriaens is thanked for synthesizing the FGG peptide. Research was funded by ERC grant 204554—SupraChemBio and CTMM—03O-201 Mammoth.


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At the double: Cucurbit[8]uril-mediated protein dimerization enables reversible control over strong enzyme activation of caspases. Simple addition of a short N-terminal FGG motif allows for a supramolecular-mediated 50-fold enhancement of caspase-9 catalytic activity.