These authors contributed equally to the work.
Polymer Nanofiber-Embedded Microchips for Detection, Isolation, and Molecular Analysis of Single Circulating Melanoma Cells†
Article first published online: 21 FEB 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 12, pages 3379–3383, March 18, 2013
How to Cite
Hou, S., Zhao, L., Shen, Q., Yu, J., Ng, C., Kong, X., Wu, D., Song, M., Shi, X., Xu, X., OuYang, W.-H., He, R., Zhao, X.-Z., Lee, T., Brunicardi, F. C., Garcia, M. A., Ribas, A., Lo, R. S. and Tseng, H.-R. (2013), Polymer Nanofiber-Embedded Microchips for Detection, Isolation, and Molecular Analysis of Single Circulating Melanoma Cells . Angew. Chem. Int. Ed., 52: 3379–3383. doi: 10.1002/anie.201208452
This research was supported by the National Institutes of Health (R21 CA151159 and R33 CA157396). Dr. F. Charles Brunicardi, Dr. Antoni Ribas, Dr. Roger S. Lo, and Dr. Hsian-Rong Tseng are members of UCLA Josson Comprehensive Cancer Center. They acknowledge generous support from JCCC and promotion of the collaboration.
- Issue published online: 13 MAR 2013
- Article first published online: 21 FEB 2013
- Manuscript Revised: 20 DEC 2012
- Manuscript Received: 20 OCT 2012
- National Institutes of Health. Grant Numbers: R21 CA151159, R33 CA157396
- cancer diagnosis;
- circulating tumor cells;
- single-cell analysis
Confined to one cell: A method to detect and isolate single circulating melanoma cells (CMCs; see figure) has been produced by integrating a polymer-nanofiber-embedded nanovelcro cell-affinity assay with a laser microdissection (LMD) technique. This method is able to separate CMCs from normal white blood cells (WBCs) and sequence individual cells for a specific mutation related to cancer progression, allowing for more personalized cancer therapy.