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Polymer Nanofiber-Embedded Microchips for Detection, Isolation, and Molecular Analysis of Single Circulating Melanoma Cells

Authors

  • Dr. Shuang Hou,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA) http://www.tseng-lab.com
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    • These authors contributed equally to the work.

  • Dr. Libo Zhao,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA) http://www.tseng-lab.com
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    • These authors contributed equally to the work.

  • Qinglin Shen,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA) http://www.tseng-lab.com
    2. Department of Applied Physics and Department of Oncology Surgery, Wuhan University, Wuhan (P.R. China)
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    • These authors contributed equally to the work.

  • Dr. Juehua Yu,

    1. Department of Surgery, University of California, Los Angeles (USA)
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    • These authors contributed equally to the work.

  • Charles Ng,

    1. Division of Hematology and Oncology, Department of Medicine, Department of Surgery, and Department of Molecular and Medical Pharmacology, University of California, Los Angeles (USA)
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  • Xiangju Kong,

    1. Division of Dermatology, Department of Medicine, University of California, Los Angeles
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  • Dr. Dongxia Wu,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA) http://www.tseng-lab.com
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  • Dr. Min Song,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA) http://www.tseng-lab.com
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  • Dr. Xiaohong Shi,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA) http://www.tseng-lab.com
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  • Xiaochun Xu,

    1. CytoLumina Technologies Corp. 21038 Commerce Point Dr., Walnut, CA 91789 (USA)
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  • Wei-Han OuYang,

    1. CytoLumina Technologies Corp. 21038 Commerce Point Dr., Walnut, CA 91789 (USA)
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  • Rongxian He,

    1. Department of Applied Physics and Department of Oncology Surgery, Wuhan University, Wuhan (P.R. China)
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  • Prof. Xing-Zhong Zhao,

    1. Department of Applied Physics and Department of Oncology Surgery, Wuhan University, Wuhan (P.R. China)
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  • Tom Lee,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA) http://www.tseng-lab.com
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  • Prof. F. Charles Brunicardi,

    1. Department of Surgery, University of California, Los Angeles (USA)
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  • Dr. Mitch André Garcia,

    Corresponding author
    1. CytoLumina Technologies Corp. 21038 Commerce Point Dr., Walnut, CA 91789 (USA)
    • CytoLumina Technologies Corp. 21038 Commerce Point Dr., Walnut, CA 91789 (USA)
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  • Prof. Antoni Ribas,

    Corresponding author
    1. Division of Hematology and Oncology, Department of Medicine, Department of Surgery, and Department of Molecular and Medical Pharmacology, University of California, Los Angeles (USA)
    • Division of Hematology and Oncology, Department of Medicine, Department of Surgery, and Department of Molecular and Medical Pharmacology, University of California, Los Angeles (USA)
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  • Prof. Roger S. Lo,

    Corresponding author
    1. Division of Dermatology, Department of Medicine, University of California, Los Angeles
    • Division of Dermatology, Department of Medicine, University of California, Los Angeles
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  • Prof. Hsian-Rong Tseng

    Corresponding author
    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA) http://www.tseng-lab.com
    • Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA) http://www.tseng-lab.com
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  • This research was supported by the National Institutes of Health (R21 CA151159 and R33 CA157396). Dr. F. Charles Brunicardi, Dr. Antoni Ribas, Dr. Roger S. Lo, and Dr. Hsian-Rong Tseng are members of UCLA Josson Comprehensive Cancer Center. They acknowledge generous support from JCCC and promotion of the collaboration.

Abstract

original image

Confined to one cell: A method to detect and isolate single circulating melanoma cells (CMCs; see figure) has been produced by integrating a polymer-nanofiber-embedded nanovelcro cell-affinity assay with a laser microdissection (LMD) technique. This method is able to separate CMCs from normal white blood cells (WBCs) and sequence individual cells for a specific mutation related to cancer progression, allowing for more personalized cancer therapy.

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