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Combinatorial Domain Swaps Provide Insights into the Rules of Fungal Polyketide Synthase Programming and the Rational Synthesis of Non-Native Aromatic Products


  • An authentic sample of pannorin was generously provided by Prof. Keiji Hasumi (Tokyo University of Agriculture and Technology, Tokyo (Japan)). This work was supported by NIH Grant ES001670.


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Playing by the rules: Combinatorial domain swaps among “deconstructed” non-reducing polyketide synthases (NR-PKSs) revealed the rules behind product assembly (see scheme). The control exerted by individual catalytic domains was found to be sufficiently great that heterocombinations of domains from different NR-PKSs synthesized products in a predictable manner.