Get access

Rapid Chemical Characterization of Bacterial Terpene Synthases

Authors

  • Patrick Rabe,

    1. Institut für Organische Chemie, Technische Universität Braunschweig, Hagenring 30, 38106 Braunschweig (Germany)
    Search for more papers by this author
  • Dr. Jeroen S. Dickschat

    Corresponding author
    1. Institut für Organische Chemie, Technische Universität Braunschweig, Hagenring 30, 38106 Braunschweig (Germany)
    • Institut für Organische Chemie, Technische Universität Braunschweig, Hagenring 30, 38106 Braunschweig (Germany)
    Search for more papers by this author

  • Funding by the Deutsche Forschungsgemeinschaft DFG with an Emmy Noether fellowship (to J.S.D., DI1536/1-1) and a grant “Duftstoffe aus Actinomyceten” (DI1536/2-1), and by the Beilstein Institut zur Förderung der Chemischen Wissenschaften with a scholarship (to P.R.) is gratefully acknowledged. We thank Stefan Schulz for support of our group.

Abstract

original image

Modern sequencing techniques deliver more and more genetic information about bacterial terpene cyclases. Chemical characterization of these enzymes must stay up to date with these developments. For this purpose a rapid and efficient method for the characterization of bacterial terpene cyclases by heterologous expression in E. coli and direct headspace analysis was developed. The products of six bacterial terpene cyclases have been identified (see structures).

Ancillary