These authors contributed equally to this work.
A Protein-Interaction Array Inside a Living Cell†
Article first published online: 4 MAR 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 18, pages 4790–4794, April 26, 2013
How to Cite
Gandor, S., Reisewitz, S., Venkatachalapathy, M., Arrabito, G., Reibner, M., Schröder, H., Ruf, K., Niemeyer, C. M., Bastiaens, P. I. H. and Dehmelt, L. (2013), A Protein-Interaction Array Inside a Living Cell . Angew. Chem. Int. Ed., 52: 4790–4794. doi: 10.1002/anie.201209127
We thank Sven Müller, Michael Schulz, Anette Langerak, Jutta Luig for technical support and Prof. Bruno Pignataro (Università di Palermo) for generous support and helpful discussions. This work was supported by BMBF, grant 0315258 to L.D., a IMPRS fellowship to S.R., a Max-Planck Fellowship to C.M.N., a Scuola Superiore di Catania fellowship to G.A., a DAAD-Siemens scholarship to M.V., and the Centre for Systems Biology in Dortmund (co-financed by the European Regional Development Fund and the State of North Rhine-Westfalia). Competing financial interests: S.G., S.R., G.A., H.S., C.M.N., L.D. and P.I.H.B. are inventors on a MPG/TU Dortmund patent application.
- Issue published online: 21 APR 2013
- Article first published online: 4 MAR 2013
- Manuscript Received: 14 NOV 2012
- Funded Access
- BMBF. Grant Number: 0315258
- Scuola Superiore di Catania
- European Regional Development Fund
- State of North Rhine-Westfalia
- multiplexed assay;
- protein-protein interactions;
- signal transduction;
Taking the bait: Protein-interaction arrays were generated in living cells by the interaction of bait-presenting artificial receptor constructs (bait-PARCs) with micrometer-scaled antibody surface patterns (see figure). This method was applied to simultaneously monitor the interaction kinetics of a prey protein with two distinct bait proteins in individual living cells.