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A Protein-Interaction Array Inside a Living Cell

Authors

  • M. Sc. Silke Gandor,

    1. Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund (Germany)
    2. Chemische Biologie, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
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    • These authors contributed equally to this work.

  • Dr. Stephanie Reisewitz,

    1. Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
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    • These authors contributed equally to this work.

  • M. Tech. Muthukumaran Venkatachalapathy,

    1. Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund (Germany)
    2. Chemische Biologie, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
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  • Dr. Giuseppe Arrabito,

    1. Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
    2. Scuola Superiore di Catania, Via Valdisavoia 9, 95123 Catania (Italy)
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  • Martina Reibner,

    1. Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
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  • Dr. Hendrik Schröder,

    1. Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
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  • M. Sc. Katharina Ruf,

    1. Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund (Germany)
    2. Chemische Biologie, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
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  • Prof. Christof M. Niemeyer,

    1. Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
    2. Institute for Biological Interfaces (IBG 1), Karlsruher Institut für Technologie (KIT), Hermann-von-Helmholtz-Platz, 76344 Eggenstein-Leopoldshafen (Germany)
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  • Prof. Philippe I. H. Bastiaens,

    Corresponding author
    1. Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund (Germany)
    2. Chemische Biologie, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
    • Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund (Germany)
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  • Dr. Leif Dehmelt

    Corresponding author
    1. Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund (Germany)
    2. Chemische Biologie, Technische Universität Dortmund, Otto-Hahn-Straße 6, 44227 Dortmund (Germany)
    • Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund (Germany)
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  • We thank Sven Müller, Michael Schulz, Anette Langerak, Jutta Luig for technical support and Prof. Bruno Pignataro (Università di Palermo) for generous support and helpful discussions. This work was supported by BMBF, grant 0315258 to L.D., a IMPRS fellowship to S.R., a Max-Planck Fellowship to C.M.N., a Scuola Superiore di Catania fellowship to G.A., a DAAD-Siemens scholarship to M.V., and the Centre for Systems Biology in Dortmund (co-financed by the European Regional Development Fund and the State of North Rhine-Westfalia). Competing financial interests: S.G., S.R., G.A., H.S., C.M.N., L.D. and P.I.H.B. are inventors on a MPG/TU Dortmund patent application.

Abstract

original image

Taking the bait: Protein-interaction arrays were generated in living cells by the interaction of bait-presenting artificial receptor constructs (bait-PARCs) with micrometer-scaled antibody surface patterns (see figure). This method was applied to simultaneously monitor the interaction kinetics of a prey protein with two distinct bait proteins in individual living cells.

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