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Discovery of Hepatitis C Virus NS3 Helicase Inhibitors by a Multiplexed, High-Throughput Helicase Activity Assay Based on Graphene Oxide


  • This work was supported by the Basic Science Research Program (2011-0017356 for D.-H. M., 2009-0092818, 2011-0020322 for B.-S. C. and 2010-0019306, 2011-0016385 for D.-E. K.), the EPB Center (2008-0062042), and by the Research Center Program (EM1202) of the Institute for Basic Science (D.-H. M.) through the National Research Foundation of Korea (NRF) funded by the Korean government (MEST).


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A GO-to solution: A simple graphene oxide (GO)-based assay to screen for selective inhibitors of a hepatitis C virus (HCV) helicase along with inhibitors of a severe acute respiratory syndrome coronavirus (SARS CoV) helicase was tested (see scheme). A single screen found five inhibitors highly selective for the HCV helicase orthologous to the SARS CoV helicase. Some of these hits were validated using the same GO-based assay.