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Keywords:

  • arginine;
  • histone deacetylases;
  • NMR spectroscopy;
  • protein dynamics;
  • spin relaxation
Thumbnail image of graphical abstract

Arginine side-chains play a distinct role because of their high pKa and perpetual positive charge. An NMR method is presented, based on carbon-detected 13Cζ15Nε correlation spectra, which allows probing the arginine side-chains and their dynamics at neutral-to-high pH. The methodology is demonstrated on human histone deacetylase 8.