Synthesis of Stable Peptide Nucleic Acid-Modified Gold Nanoparticles and their Assembly onto Gold Surfaces


  • This work was supported by the Swiss National Science Foundation (Professorship No. PP00P2_133568 to G.G.), the University of Zurich (G.G.), the Australian Research Council (Australian Research Fellowship to U.B. and Future Fellowship to A.M.F. (FT110100545)) and the Commonwealth Scientific and Industrial Research Organization (OCE Science Leader position to U.B.). This work was performed in part at the Melbourne Centre for Nanofabrication, an initiative partly funded by the Commonwealth of Australia and the Victorian Government.


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PNA does it better: Peptide nucleic acid (PNA) oligomers were attached to gold nanoparticles (AuNPs) through a variety of mono- and trithiol linkers. These functionalized particles had sufficient stability for sequence-specific self-assembly onto gold surfaces (see figure) in the absence of ions or surfactants. The nanoparticle surface densities obtained were superior to comparable DNA-modified AuNPs.