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Towards Structure Determination of Self-Assembled Peptides Using Dynamic Nuclear Polarization Enhanced Solid-State NMR Spectroscopy

Authors

  • Dr. Hiroki Takahashi,

    1. Laboratoire de Chimie Inorganique et Biologique, UMR-E3 (CEA/UJF) and CNRS, Institut Nanosciences et Cryogénie, CEA, 38054 Grenoble (France)
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  • Bastien Viverge,

    1. Laboratoire de Chimie Inorganique et Biologique, UMR-E3 (CEA/UJF) and CNRS, Institut Nanosciences et Cryogénie, CEA, 38054 Grenoble (France)
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  • Dr. Daniel Lee,

    1. Laboratoire de Chimie Inorganique et Biologique, UMR-E3 (CEA/UJF) and CNRS, Institut Nanosciences et Cryogénie, CEA, 38054 Grenoble (France)
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  • Dr. Patrice Rannou,

    1. Laboratoire d'Electronique Moléculaire, Organique et Hybride, UMR5819-SPrAM, Institut Nanosciences et Cryogénie, CEA, 38054 Grenoble (France)
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  • Dr. Gaël De Paëpe

    Corresponding author
    1. Laboratoire de Chimie Inorganique et Biologique, UMR-E3 (CEA/UJF) and CNRS, Institut Nanosciences et Cryogénie, CEA, 38054 Grenoble (France)
    • Laboratoire de Chimie Inorganique et Biologique, UMR-E3 (CEA/UJF) and CNRS, Institut Nanosciences et Cryogénie, CEA, 38054 Grenoble (France)

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  • This work was supported by the French National Research Agency through the “programme blanc” (grant number ANR-12-BS08-0016-01) and the “programme Labex” (ARCANE project number ANR-11-LABX-003) and funding from the RTB. SEM images were taken at the PFNC platform at MINATEC (CEA Grenoble). P. A. Bayle is acknowledged for solution NMR measurements.

Abstract

original image

Supra-sensitivity: Dynamic nuclear polarization (DNP) enhanced solid-state NMR spectroscopy was performed on self-assembled peptide nanotubes. This approach yields significant experimental time savings (about five orders of magnitude; see picture) and was used to exemplify the feasibility of supramolecular structural studies of organic nanoassemblies at an atomic scale using DNP-enhanced solid-state NMR spectroscopy.

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