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Formation of 1,3-Cyclohexanediones and Resorcinols Catalyzed by a Widely Occuring Ketosynthase

Authors

  • Dipl.-Biol. Sebastian W. Fuchs,

    1. Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
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  • Dipl.-Bioinf. Kenan A. J. Bozhüyük,

    1. Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
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  • Dipl.-Bioinf. Darko Kresovic,

    1. Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
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  • Florian Grundmann,

    1. Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
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  • M. Sc. Veronica Dill,

    1. Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
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  • Dr. Alexander O. Brachmann,

    1. Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
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  • Dr. Nicholas R. Waterfield,

    1. Department of Biology and Biochemistry, Building 3 South, Room 0.28, University of Bath, Claverton Down, Bath BA2 7AY (UK)
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  • Prof. Dr. Helge B. Bode

    Corresponding author
    1. Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
    • Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Str. 9, 60438 Frankfurt am Main (Germany)
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  • This work was funded by the Deutsche Forschungsgemeinschaft (DFG). We thank Harry R. Beller and Tim Schöner for providing plasmids and enzymes, Peter Grün for help with compound purification, Imke Schmitt and Francesco Dal Grande for computing time for phylogenetic analysis, and Michael Karas for MALDI access.

Abstract

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Overlooked, but widespread! A new class of ketosynthases (DarB) involved in the biosynthesis of 1,3-cyclohexanediones and dialkylresorcinols has been identified and characterized in detail. The presence of homologues in 89 different bacteria, including several pathogens, reveals that DarB as well as the corresponding natural products might be widespread, thus presenting a new but so far overlooked pathway to natural products.

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