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Studying 18 MDa Virus Assemblies with Native Mass Spectrometry

Authors

  • Joost Snijder,

    1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences and Netherlands Proteomics Centre, Utrecht University, Padualaan 8, 3584 CH, Utrecht (The Netherlands) http://bioms.chem.uu.nl
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  • Dr. Rebecca J. Rose,

    1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences and Netherlands Proteomics Centre, Utrecht University, Padualaan 8, 3584 CH, Utrecht (The Netherlands) http://bioms.chem.uu.nl
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  • Dr. David Veesler,

    1. Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037 (USA)
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  • Prof. Dr. John E. Johnson,

    1. Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037 (USA)
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  • Prof. Dr. Albert J. R. Heck

    Corresponding author
    1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences and Netherlands Proteomics Centre, Utrecht University, Padualaan 8, 3584 CH, Utrecht (The Netherlands) http://bioms.chem.uu.nl
    • Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences and Netherlands Proteomics Centre, Utrecht University, Padualaan 8, 3584 CH, Utrecht (The Netherlands) http://bioms.chem.uu.nl
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Errata

This article is corrected by:

  1. Errata: Corrigendum: Studying 18 MDa Virus Assemblies with Native Mass Spectrometry Volume 53, Issue 12, 3051, Article first published online: 14 March 2014

  • This project was supported by grants from the NIH (R01 AI040101) and by a FP7 Marie-Curie IOF fellowship (273427 to D.V.).

Abstract

original image

Setting records: Native mass spectometry was used to study the assembly of the 18 MDa capsid of bacteriophage HK97. The previous record for the analysis of capsids by this method was around 10 MDa. The results indicate that the efficiency of desolvation is the main priority in improving native MS instrumentation.

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