Red Fluorescent Probe for Monitoring the Dynamics of Cytoplasmic Calcium Ions


  • This research was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan (Specially Promoted Research Grant No. 22000006 to T.N.), SENTAN, the JST (grant to K.H.), and the Funding Program for Next Generation World-Leading Researchers (LS023 to Y.I.). K.H. was also supported by grants from the Inoue Foundation for Science, the Takeda Science Foundation, the Tokyo Biochemical Research Foundation, and the Astellas Foundation for Research on Metabolic Disorders.


original image

We see red (and yellow and green): Probe 1 was developed for the visualization of cytoplasmic Ca2+, a pivotal second messenger in many biological responses. The new probe is suitable for multicolor imaging for the simultaneous detection of metal ions or proteins and is superior to the existing red fluorescent probe Rhod-2 for the monitoring of cytoplasmic Ca2+ oscillation in cultured cells (see fluorescence images of cells with 1 (top) and Rhod-2 (bottom)).