Suppression of Gene Expression by G-Quadruplexes in Open Reading Frames Depends on G-Quadruplex Stability

Authors

  • Tamaki Endoh,

    1. Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Kobe 650-0047 (Japan)
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  • Yu Kawasaki,

    1. Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Kobe 650-0047 (Japan)
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  • Prof. Naoki Sugimoto

    Corresponding author
    1. Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Kobe 650-0047 (Japan)
    • Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Kobe 650-0047 (Japan)

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  • Customized PUREfrex was kindly provided from GeneFrontier Corp. (http://www.genefrontier.com/english/index.html). This work was supported in part by Grants-in-Aid for Scientific Research and MEXT (Japan)-Supported Program for the Strategic Research Foundation at Private Universities (2009–2014).

Abstract

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Suppressing the urge to translate: Sequences with the potential to form G-quadruplexes were identified in the open reading frames of E. coli genes. These sequences were found to form parallel G-quadruplexes and to suppress translation (see picture) of the mRNAs into proteins both in vitro and in cells.

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