Distinguishing Alternative Reaction Pathways by Single-Molecule Fluorescence Spectroscopy


  • We thank the Deutsche Forschungsgemeinschaft (DFG) for their financial support (EXC81, SFB623). We also acknowledge Stephen Hashmi (Heidelberg University) for fruitful discussions. Volker Huch is gratefully acknowledged for X-ray crystallography. Michael Schwering and Dominik Brox have continuously supported the project with their expertise in microscopy.


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Focus on chemical transitions: Epoxidation of a double bond in conjugation to a fluorescent dye was studied at single-molecule level. Direct observation of oxirane formation, indicated as a spectral shift from substrate to product state, revealed an alternative reaction pathway for the epoxidation reaction.