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Steady-State Continuous-Flow Purification by Electrophoresis

Authors

  • Fletcher J. Agostino,

    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
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  • Dr. Leonid T. Cherney,

    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
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  • Dr. Victor Galievsky,

    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
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  • Prof. Sergey N. Krylov

    Corresponding author
    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
    • Department of Chemistry and Centre for Research on Biomolecular Interactions, York University, 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)

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  • We thank Natural Sciences and Engineering Research Council of Canada.

Abstract

original image

Bubble and sweep: Free-flow electrophoresis (FFE) could allow continuous-flow purification of products from continuous-flow microsynthesis. However, gas bubbles distort the separation. An open-electrolyte FFE system avoids this problem. Electrodes placed in chimneys open to the atmosphere mean bubbles are vented directly from the system and cannot enter the separation channel (see picture).

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