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Molecular Imaging of Cancer Cells Using a Bacteriophage-Based 129Xe NMR Biosensor

Authors

  • Dr. Krishnan K. Palaniappan,

    1. Department of Chemistry, University of California, Berkeley, CA 94720-1460 (USA)
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    • These authors contributed equally to this work.

  • R. Matthew Ramirez,

    1. Department of Chemistry, University of California, Berkeley, CA 94720-1460 (USA)
    2. Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-1460 (USA)
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    • These authors contributed equally to this work.

  • Dr. Vikram S. Bajaj,

    1. Department of Chemistry, University of California, Berkeley, CA 94720-1460 (USA)
    2. Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-1460 (USA)
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  • Prof. Dr. David E. Wemmer,

    1. Department of Chemistry, University of California, Berkeley, CA 94720-1460 (USA)
    2. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-1460 (USA)
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  • Prof. Dr. Alexander Pines,

    1. Department of Chemistry, University of California, Berkeley, CA 94720-1460 (USA)
    2. Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-1460 (USA)
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  • Prof. Dr. Matthew B. Francis

    Corresponding author
    1. Department of Chemistry, University of California, Berkeley, CA 94720-1460 (USA)
    2. Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-1460 (USA)
    • Department of Chemistry, University of California, Berkeley, CA 94720-1460 (USA)
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  • This work was supported by grants from the U.S. Department of Defense Cancer Research Program (grant number BC016995, M.B.F.) and by the U.S. Department of Energy, Office of Basic Energy Sciences, Division of Materials Science and Engineering under contract number DE-AC02-05CH112 (A.P.).

Abstract

original image

NMR imaging: Filamentous fd bacteriophage expressing antibodies recognizing the epidermal growth factor receptor (EGFR) were modified to incorporate cage-like xenon-binding molecules (CryA). The resulting contrast agent was shown to bind to an EGFR-positive cell line and detected by hyperpolarized 129Xe NMR spectroscopy using chemical exchange saturation transfer (hyperCEST, see picture).

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