Integrated Bare Narrow Capillary–Hydrodynamic Chromatographic System for Free-Solution DNA Separation at the Single-Molecule Level

Authors

  • Zaifang Zhu,

    1. Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 (USA)
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  • Huang Chen,

    1. Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 (USA)
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  • Dr. Wei Wang,

    1. Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 (USA)
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  • Aaron Morgan,

    1. Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 (USA)
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  • Dr. Congying Gu,

    1. Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 (USA)
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  • Prof. Chiyang He,

    Corresponding author
    1. College of Chemistry and Chemical Engineering, Wuhan Textile University, Wuhan, Hubei 430073 (P.R. China)
    • Chiyang He, College of Chemistry and Chemical Engineering, Wuhan Textile University, Wuhan, Hubei 430073 (P.R. China)

      Shaorong Liu, Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 (USA)

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  • Joann J. Lu,

    1. Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 (USA)
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  • Prof. Shaorong Liu

    Corresponding author
    1. Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 (USA)
    • Chiyang He, College of Chemistry and Chemical Engineering, Wuhan Textile University, Wuhan, Hubei 430073 (P.R. China)

      Shaorong Liu, Department of Chemistry and Biochemistry, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019 (USA)

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  • We thank Department of Energy (DE-SC0006351), National Science Foundation (CHE 1011957), and the Oklahoma Center for the Advancement of Science and Technology (AR11-003) for support.

Abstract

original image

Turn the volume down: Coupling a high-pressure electroosmotic pump (EOP) and a microfabricated chip-injector with a bare narrow capillary–hydrodynamic chromatographic system (BaNC-HDC), enables samples to be injected at low-picoliter volumes, analytes to be eluted at picoliters per minute, and a wide size range of DNA fragments to be resolved (see picture) rapidly in free solution at the single-molecule level.

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