These authors contributed equally to this work.
A Genetically Encoded 19F NMR Probe for Tyrosine Phosphorylation†
Article first published online: 28 FEB 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Angewandte Chemie International Edition
Volume 52, Issue 14, pages 3958–3962, April 2, 2013
How to Cite
Li, F., Shi, P., Li, J., Yang, F., Wang, T., Zhang, W., Gao, F., Ding, W., Li, D., Li, J., Xiong, Y., Sun, J., Gong, W., Tian, C. and Wang, J. (2013), A Genetically Encoded 19F NMR Probe for Tyrosine Phosphorylation . Angew. Chem. Int. Ed., 52: 3958–3962. doi: 10.1002/anie.201300463
We gratefully acknowledge the Major State Basic Research Program of China (grant numbers 2010CB912301 and 2009CB825505), the National Science Foundation of China (grant numbers 90913022, 31000364, 31170817, and 31100563), the CAS grant (grant number KSCX2-EW-G-7) to J.W., F.L., C.T., and Y.X. We thank Professors Zongchao Jia and Jingxuan Pan for helpful discussion.
- Issue published online: 26 MAR 2013
- Article first published online: 28 FEB 2013
- Manuscript Received: 18 JAN 2013
- Major State Basic Research Program of China. Grant Numbers: 2010CB912301, 2009CB825505
- National Science Foundation of China. Grant Numbers: 90913022, 31000364, 31170817, 31100563
- CAS. Grant Number: KSCX2-EW-G-7
- NMR spectroscopy;
- protein–protein interactions;
- tyrosine kinase;
- tyrosine phosphorylation
Simple and selective: Tyrosine phosphorylation is a pivotal post-translational modification which regulates the enzymatic activity, protein conformation, and protein–protein interactions. The highly efficient genetic incorporation of 3,5-difluorotyrosine (F2Y) in E. coli and the use of F2Y as a 19F NMR probe for the tyrosine phosphorylation are reported (see picture).