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Keywords:

  • NMR spectroscopy;
  • protein–protein interactions;
  • proteins;
  • tyrosine kinase;
  • tyrosine phosphorylation
Thumbnail image of graphical abstract

Simple and selective: Tyrosine phosphorylation is a pivotal post-translational modification which regulates the enzymatic activity, protein conformation, and protein–protein interactions. The highly efficient genetic incorporation of 3,5-difluorotyrosine (F2Y) in E. coli and the use of F2Y as a 19F NMR probe for the tyrosine phosphorylation are reported (see picture).