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Keywords:

  • phosphoserine;
  • unnatural amino acids;
  • histones;
  • phosphorylation;
  • protein engineering
Thumbnail image of graphical abstract

Phosphoserine incorporation: A general strategy for producing recombinant histones with site-specific serine phosphorylation is developed by engineering phosphoseryl-tRNA synthetase (SepRS) and elongation factor Tu (EF-Tu; see picture). Serine-phosphorylated nucleosomes provide direct evidence for crosstalk between phosphorylation and acetylation in histones.