Conformational Transitions at an S-Layer Growing Boundary Resolved by Cryo-TEM

Authors


  • This work was performed at Lawrence Berkeley National Laboratory, with support from the Office of Science, Office of Basic Energy Sciences, Biological and Environmental Research, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231.

Abstract

original image

Snapshots of growth: The use of a single graphene layer as the support for S-layer self-assembly of proteins provides an ideal, robust flat surface to image the expanding boundary by flash freezing and cryo-TEM, capturing the conformational states present at the expanding boundary (see figure). Image alignment and averaging provide a view of the steps leading to subunit recruitment and maturation in the self-assembly process.

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