Development of a MALDI-TOF MS Strategy for the High-Throughput Analysis of Biomarkers: On-Target Aptamer Immobilization and Laser-Accelerated Proteolysis

Authors

  • Xueyang Zhang,

    1. Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai,200433 (China)
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  • Shaochun Zhu,

    1. Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai,200433 (China)
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  • Ya Xiong,

    1. Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai,200433 (China)
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  • Prof. Dr. Chunhui Deng,

    Corresponding author
    1. Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai,200433 (China)
    • Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai,200433 (China)

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  • Prof. Dr. Xiangmin Zhang

    Corresponding author
    1. Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai,200433 (China)
    • Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai,200433 (China)

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  • This work was supported by the National Basic Research Priorities Program (2013CB911201, 2012CB910602), 863 Project (2012AA020202), the National Natural Science Foundation of China (21075022, 21275033, 21105016), a Research Fund for the Doctoral Program of Higher Education of China (20110071110007), and the Shanghai Leading Academic Discipline Project (B109).

Abstract

original image

An aptamer-based strategy was developed for the high-throughput analysis of protein biomarkers, such as lysozyme, by on-target MALDI-TOF MS. The aptamers were immobilized on the target plate through formation of covalent bonds with a stable and porous gold layer. An infrared laser was subsequently applied for fast proteolysis (see picture). High sensitivities were observed both in standard solutions and human urine.

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