Thermo-Responsive Hydrogel Layers Imprinted with RGDS Peptide: A System for Harvesting Cell Sheets

Authors

  • Dr. Guoqing Pan,

    Corresponding author
    1. Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu 215006 (China)
    2. Orthopaedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215007 (China)
    • Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu 215006 (China)

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  • Qianping Guo,

    1. Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu 215006 (China)
    2. Orthopaedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215007 (China)
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  • Yue Ma,

    1. Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Department of Chemistry, Nankai University, Tianjin 300071 (China)
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  • Prof. Dr. Huilin Yang,

    1. Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu 215006 (China)
    2. Orthopaedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215007 (China)
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  • Prof. Dr. Bin Li

    Corresponding author
    1. Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu 215006 (China)
    2. Orthopaedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215007 (China)
    • Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu 215006 (China)

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  • We gratefully acknowledge financial support from the National Natural Science Foundation of China (21204056, 81171479), the Natural Science Foundation of Jiangsu Province (BK2012173, BK2011291), and the China Postdoctoral Science Foundation funded project (2012M520060). RGDS peptide=Arg-Gly-Asp-Ser.

Abstract

original image

The efficient promotion of cell adhesion on a polymer surface and detachment of sheets of cells is possible with thermo-responsive cell culture substrates that are biofunctionalized through noncovalent molecular imprinting. The key is the thermo-responsive “specific binding” of the cell-adhesive peptide RGDS on the cell sheet harvest system (see picture).

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