Interactions of a Periplasmic Binding Protein with a Tetradentate Siderophore Mimic


  • We thank the UK Biotechnology and Biological Sciences Research Council (BBSRC) for financial support, the Diamond Light Source for access to beamlines i03 and i24, Dr. J. Turkenburg, S. Hart for assistance with data collection, and Dr. A. Leech for help with circular dichroism spectroscopy.


original image

Iron-bound structure: The ferric complex of a tetradentate siderophore mimic was synthesized and co-crystallized with the periplasmic binding protein CeuE of Campylobacter jejuni. In addition to electrostatic and hydrogen-bonding interactions between the binding pocket and the substrate, the structure showed direct coordination of two amino acid side chains to the FeIII center (orange, see figure).