Visualization of Oxygen Consumption of Single Living Cells by Scanning Electrochemical Microscopy: The Influence of the Faradaic Tip Reaction

Authors

  • Dr. Michaela Nebel,

    1. Lehrstuhl für Analytische Chemie, Elektroanalytik & Sensorik and Center for Electrochemical Sciences, CES, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum (Germany)
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  • Stefanie Grützke,

    1. Lehrstuhl für Analytische Chemie, Elektroanalytik & Sensorik and Center for Electrochemical Sciences, CES, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum (Germany)
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  • Prof. Dr. Nizam Diab,

    1. Chemistry Department; Faculty of Arts and Sciences, The Arab American University, P.O. Box 240, Jenin (Palestine)
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  • Prof. Dr. Albert Schulte,

    1. Biochemistry-Electrochemistry Research Unit, Schools of Chemistry and Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000 (Thailand)
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  • Prof. Dr. Wolfgang Schuhmann

    Corresponding author
    1. Lehrstuhl für Analytische Chemie, Elektroanalytik & Sensorik and Center for Electrochemical Sciences, CES, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum (Germany)
    • Lehrstuhl für Analytische Chemie, Elektroanalytik & Sensorik and Center for Electrochemical Sciences, CES, Ruhr-Universität Bochum, Universitätsstrasse 150, 44780 Bochum (Germany)

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  • The authors are grateful to Dr. Dominik Hollatz, Sven Zielke, and Dr. Christian H. Wetzel (Lehrstuhl für Zellphysiologie, Ruhr-Universität Bochum) for the supply of the cell samples, and to Dr. Sonnur Isik-Uppenkamp for her contribution during the initial stage of this project. This work was supported by the EU and the state NRW in the framework of the HighTech.NRW program. M.N. thanks the Ruhr-University Research School funded by Germany’s Excellence Initiative for further support [DFG GSC98/1].

Abstract

original image

The influence of the reaction rate at the SECM tip on the overall imaging result is often neglected during respiration studies performed by SECM. The effect of the driving force of the tip reaction is elucidated using a potential pulse profile implemented into a constant-distance mode. Time-dependent data acquisition allows visualization of the transition between a tip behaving as a passive observer and a tip actively inducing transmembrane diffusion of oxygen.

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