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Virus-Mimetic Cytoplasm-Cleavable Magnetic/Silica Nanoclusters for Enhanced Gene Delivery to Mesenchymal Stem Cells

Authors

  • Naveen Gandra,

    1. Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Room 3310, Norman, OK 73019-5300 (USA)
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    • These authors contributed equally to this work.

  • Dong-Dong Wang,

    1. Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Room 3310, Norman, OK 73019-5300 (USA)
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    • These authors contributed equally to this work.

  • Ye Zhu,

    1. Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Room 3310, Norman, OK 73019-5300 (USA)
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  • Prof. Chuanbin Mao

    Corresponding author
    1. Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Room 3310, Norman, OK 73019-5300 (USA)
    • Department of Chemistry & Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, 101 Stephenson Parkway, Room 3310, Norman, OK 73019-5300 (USA)

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  • We would like to thank the National Science Foundation (CBET-0854465, CBET-0854414 and DMR-0847758), National Institutes of Health (4R03AR056848-03, 1R21EB015190-02, and 5R01HL092526-02), Department of Defense Peer Reviewed Medical Research Program (W81XWH-12-1-0384), Oklahoma Center for the Advancement of Science and Technology (HR11-006), and Oklahoma Center for Adult Stem Cell Research (434003) for their generous financial support. We also want to thank Dr. Haibao Zhu and Dr. Gopal Abbineni for their kind help during experiments.

Abstract

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It does get in: Phage is made of DNA as a core and protein as a coat, and it can transfer DNA into host cells with high efficiency. Phage-mimetic gene transfer to hard-to-transfect mesenchymal stem cells (MSCs) was achieved using virus-mimetic magnetic silica nanoclusters (VMSNCs). The VMSNCs bear MSC-homing phage-borne protein on the surface and encapsulate DNA inside, promoting the transfer of DNA into MSCs.

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