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Structure and Reaction Mechanism of Pyrrolysine Synthase (PylD)

Authors

  • Felix Quitterer,

    1. Center for Integrated Protein Science Munich (CIPSM) at the Department Chemie, Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)
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  • Philipp Beck,

    1. Center for Integrated Protein Science Munich (CIPSM) at the Department Chemie, Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)
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  • Prof. Dr. Adelbert Bacher,

    1. Center for Integrated Protein Science Munich (CIPSM) at the Department Chemie, Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)
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  • Prof. Dr. Michael Groll

    Corresponding author
    1. Center for Integrated Protein Science Munich (CIPSM) at the Department Chemie, Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)
    • Center for Integrated Protein Science Munich (CIPSM) at the Department Chemie, Lehrstuhl für Biochemie, Technische Universität München, Lichtenbergstrasse 4, 85747 Garching (Germany)

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  • We thank the staff of the beamline X06SA at the Paul Scherrer Institute, Swiss Light Source, Villigen (Switzerland) for their help with data collection and Katrin Gärtner for excellent technical assistance. We acknowledge Dr. Anja List who participated in the PylD project. This work was supported by the Hans-Fischer-Gesellschaft, by Award No. FIC/2010/07 from the King Abdullah University of Science and Technology (KAUST), and by the Deutsche Forschungsgemeinschaft (DFG, grant GR1861/7-1).

Abstract

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The final step in the biosynthesis of the 22nd genetically encoded amino acid, pyrrolysine, is catalyzed by PylD, a structurally and mechanistically unique dehydrogenase. This catalyzed reaction includes an induced-fit mechanism achieved by major structural rearrangements of the N-terminal helix upon substrate binding. Different steps of the reaction trajectory are visualized by complex structures of PylD with substrate and product.

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