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DNA Origami as a DNA Repair Nanosensor at the Single-Molecule Level

Authors

  • Maria Tintoré,

    1. IRB Barcelona, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering Biomaterials and Nanomedicine c/Jordi Girona 18-26, 08034 Barcelona (Spain)
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    • These authors contributed equally to this work.

  • Dr. Isaac Gállego,

    1. IRB Barcelona, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering Biomaterials and Nanomedicine c/Jordi Girona 18-26, 08034 Barcelona (Spain)
    2. Present address: School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400 (USA)
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    • These authors contributed equally to this work.

  • Dr. Brendan Manning,

    1. IRB Barcelona, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering Biomaterials and Nanomedicine c/Jordi Girona 18-26, 08034 Barcelona (Spain)
    2. Biokit, S.A. 08186 Lliçà d'Amunt, Barcelona (Spain)
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  • Dr. Ramon Eritja,

    1. IRB Barcelona, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering Biomaterials and Nanomedicine c/Jordi Girona 18-26, 08034 Barcelona (Spain)
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  • Dr. Carme Fàbrega

    Corresponding author
    1. IRB Barcelona, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering Biomaterials and Nanomedicine c/Jordi Girona 18-26, 08034 Barcelona (Spain)
    • IRB Barcelona, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering Biomaterials and Nanomedicine c/Jordi Girona 18-26, 08034 Barcelona (Spain)
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  • This work was supported by European Communities FUNMOL, “Fondo de Investigaciones Sanitarias” (grant PI06/1250) and by “Ministerio Ciencia e Innovación” (grant CTQ-2010-20541-C03-03). C.F. is grateful to Generalitat de Catalunya and Instituto de Salud Carlos III for a SNS Miguel Servet contract. We acknowledge Gerard Oncins from Scientific and Technological Centers of the University of Barcelona (CCiTUB) for his help and advice regarding AFM.

Abstract

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The folding of DNA molecules by DNA origami is used in a nanosensor to analyze enzymatic DNA repair activity of hAGT. The method uses conformational changes that condition α-thrombin interaction with DNA aptamers, and illustrates the use of DNA origami as a proteinrecognition biosensor.

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