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A Bioorthogonal Raman Reporter Strategy for SERS Detection of Glycans on Live Cells

Authors

  • Liang Lin,

    1. Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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    • These authors contributed equally to this work.

  • Xiangdong Tian,

    1. State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
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    • These authors contributed equally to this work.

  • Senlian Hong,

    1. School of Life Sciences, Peking University, Beijing 100871 (China)
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    • These authors contributed equally to this work.

  • Peng Dai,

    1. Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Qiancheng You,

    1. Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Ruyi Wang,

    1. Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Lianshun Feng,

    1. Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
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  • Prof. Can Xie,

    1. School of Life Sciences, Peking University, Beijing 100871 (China)
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  • Prof. Zhong-Qun Tian,

    Corresponding author
    1. State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)
    • Zhong-Qun Tian, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)

      Xing Chen, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)

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  • Prof. Xing Chen

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)
    • Zhong-Qun Tian, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China)

      Xing Chen, Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871 (China)

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  • This work was supported by the National Instrumentation Program under grant number2011YQ030124 (grant to Z.T. and X.C.), the National Basic Research Program of China (973 Program) under grant number 2012CB917303 (grant to X.C.), and the National Natural Science Foundation of China under grant number 21172013 (grant to X.C.). We thank Prof. C. Yan, Prof. Y. Li, and Prof. Y. Huang for sharing the instruments. SERS=surface-enhanced Raman spectroscopy.

Abstract

original image

Direct detection of glycans on live cells using surface-enhanced Raman scattering (SERS) has been shown. A bioorthogonal Raman reporter was directly installed onto the monosaccharide analogs. Once metabolically incorporated into cell surface glycans, the Raman reporter was detected using SERS (see picture).

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