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Building Fluorescent DNA Nanodevices on Target Living Cell Surfaces

Authors

  • Guizhi Zhu,

    1. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082 (China)
    2. Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200 (USA)
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  • Shengfeng Zhang,

    1. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082 (China)
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  • Prof. Dr. Erqun Song,

    1. Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200 (USA)
    2. Key Laboratory of Luminescence and Real-Time Analysis of the Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715 (China)
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  • Jing Zheng,

    1. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082 (China)
    2. Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200 (USA)
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  • Rong Hu,

    1. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082 (China)
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  • Prof. Dr. Xiaohong Fang,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Institute of Chemistry, Chinese Academy of Sciences, ZhongGuanCun North 1st Street Beijing 100190 (China)
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  • Prof. Dr. Weihong Tan

    Corresponding author
    1. Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082 (China)
    2. Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200 (USA)
    • Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082 (China)

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  • The authors sincerely thank Dr. K. R. Williams for critical manuscript review. This work is supported by grants awarded by the National Key Scientific Program of China (grant numbers 2011CB911000, and 2013CB933701), the Foundation for Innovative Research Groups of NSFC (grant number 21221003), China National Instrumentation Program (grant number 2011YQ03012412), and by the National Institutes of Health (grant numbers GM066137, GM079359, and CA133086).

Abstract

original image

DNA nanotrain: Anchoring of preformed fluorescent DNA nanodevices (NDs; see picture) and in situ self-assembly of fluorescent DNA NDs on target living cell surfaces are reported. The in situ self-assembly of the nanodevice was further shown on surfaces of living cells in cell mixtures. These DNA NDs exhibited fluorescence emission and underwent fluorescence resonance energy transfer (FRET) on living cell surfaces.

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